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Sodium phosphate buffer





Sodium phosphate - Cold Spring Harbor Protocols - Cshlp.org

10/29/2014
12:40 | Author: Caitlin White

Sodium phosphate buffer
Sodium phosphate - Cold Spring Harbor Protocols - Cshlp.org

Sodium phosphate. 1 M sodium phosphate buffer (pH 6.0–7.2). Mixing 1 M NaH2PO4 (monobasic) and 1 M Na2HPO4 (dibasic) stock solutions in the volumes.

Add 3.1 g of NaH 2 PO 4 •H 2 O and 10.9 g of Na 2 HPO 4 (anhydrous) to distilled H 2 O to make a volume of 1 L. This buffer can be stored for up to 1 mo at 4°C. The pH of the final solution will be 7.4.

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0.1 M sodium phosphate buffer (pH 7.4).

0.1 M sodium phosphate buffer (from 1 M stocks) at 25°C.

1 M sodium phosphate buffer (pH 6.0–7.2).

What's this? What's this?. To prepare 1 L of 0.1 M sodium phosphate buffer of the desired pH, the following mixtures should be diluted to 1 L (final volume) with H 2 O.

Mixing 1 M NaH 2 PO 4 (monobasic) and 1 M Na 2 HPO 4 (dibasic) stock solutions in the volumes designated in the table below results in 1 L of 1 M sodium phosphate buffer of the desired pH. To prepare the stock solutions, dissolve 138 g of NaH 2 PO 4 •H 2 O (monobasic; m.w. = 138) in sufficient H 2 O to make a final volume of 1 L and dissolve 142 g of Na 2 HPO 4 (dibasic; m.w. = 142) in sufficient H 2 O to make a final volume of 1 L.

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Phosphate Buffer Calculation (Javascript) - Fuse Home Pages

12/28/2014
10:20 | Author: Caitlin White

Cirrhosis sodium retention
Phosphate Buffer Calculation (Javascript) - Fuse Home Pages

Phosphate Buffer Calculator. A Javascript that calculates the amount of monosodium phosphate and disodium phosphate necessary to achieve a buffer at a.

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1 M Sodium Phosphate Buffer (pH 5.8-8.0)

10/27/2014
08:20 | Author: Caitlin White

Cirrhosis sodium retention
1 M Sodium Phosphate Buffer (pH 5.8-8.0)

PH. 1 M Na2HPO4. 1 M NaH2PO4. 8.0. 93.2 ml. 6.8 ml. 7.8. 89.6 ml. 10.4 ml. 7.6. 84.5 ml. 15.5 ml. 7.4. 77.4 ml. 22.6 ml. 7.2. 68.4 ml. 31.6 ml. 7.0. 57.7 ml.

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Buffer Reference Center Sigma-Aldrich

8/26/2014
06:20 | Author: Caitlin White

Sodium phosphate buffer
Buffer Reference Center Sigma-Aldrich

Potassium Phosphate Monobasic Anhydrous g/L, Sodium Phosphate Dibasic Heptahydrate g/L, 23 °C.

68.08. Imidazole (glyoxaline) – HCl buffer solutions, pH 6.2–7.8 at 25 °C 1 Imidazole,, M. wt. 25 ml 0.2M-imidazole (13.62 g/l), x ml 0.2M-HCl, diluted to 100 ml with H 2 O.

wt. Na 2 HPO 4, M. Citric Acid – Na 2 HPO 4 Buffer Solutions, pH approx. 178.05; 0.2M-solution contains 35.61 g/l. 2.6–7.6 1 Citric acid monohydrate, • H 2 O, M. 210.14; 0.1M-solution contains 21.01 g/l. wt. wt. 141.98; 0.2M-solution contains 28.40 g/l, or Na 2 HPO 4 • 2H 2 O, M. x ml 0.1M-citric acid and y ml 0.2M-Na 2 HPO 4 mixed.

178.05; 0.2M-solution contains 35.61 g/l.

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How to Make a Phosphate Buffer Solution - Chemistry - About.com

6/25/2014
04:40 | Author: Caitlin White

Sodium phosphate buffer
How to Make a Phosphate Buffer Solution - Chemistry - About.com

A phosphate buffer solution is a handy buffer solution to have around, phosphoric acid to make the pH more acidic or sodium hydroxide to.

For a 1 M buffer (selected to make the calculation easy), + = 1. The molarity of the buffer is the sum of the molarities of the acid and conjugate base or the sum of +.

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Updated May 13, 2014.

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ratio of / = 1.096.

substitute this into the ratio and solve:

Now solve for. = 1 - so = 0.477 moles/L.

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= 0.523 moles/L.

A phosphate buffer solution is a handy buffer to have around, especially for biological applications. The buffer is most commonly prepared at pH 7 using monosodium phosphate and its conjugate base, disodium phosphate. Because phosphoric acid has multiple dissociation constants, you can prepare phosphate buffers near any of the three pHs, which are at 2.15, 6.86 and 12.32.

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pH = pKa + log (/).

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