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Monobasic and dibasic sodium phosphate buffer





PB (0.1 M phosphate buffer pH 7.2) - Cold Spring Harbor Protocols

11/21/2014
09:28 | Author: Evan Martin

Sodium phosphate monobasic vs dibasic
PB (0.1 M phosphate buffer pH 7.2) - Cold Spring Harbor Protocols

Prepare sodium phosphate dibasic stock (0.5 M) by dissolving 35.5 g of M) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of.

Prepare 0.1 M sodium phosphate monobasic: Put 30 mL of sodium phosphate monobasic stock (0.5 M) from Step 2 in a beaker and add H 2 O to give a final volume of 150 mL. 4.

Prepare sodium phosphate monobasic stock (0.5 M) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of 500 mL of H 2 O. 2.

Bring the 0.1 M sodium phosphate dibasic solution from Step 3 to pH 7.2 by adding as much as needed of the 0.1 M sodium phosphate monobasic solution from Step 4. 5.

Prepare sodium phosphate dibasic stock (0.5 M) by dissolving 35.5 g of sodium phosphate dibasic in a final volume of 500 mL of H 2 O. 1.

Hot plate (optional; see Step 1 note) Scale Spatulas Stir plate Weigh boats.

What's this? What's this?. The resulting solution is 0.1 M phosphate buffer pH 7.2.

3. Prepare 0.1 M sodium phosphate dibasic: Put 80 mL of sodium phosphate dibasic stock (0.5 M) from Step 1 in a beaker and add H 2 O to give a final volume of 400 mL.

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Sodium phosphate monobasic (anhydrous) Beakers Graduated cylinders.

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Warm on a hot plate and stir until the crystals dissolve. Some crystallization will occur when the solution is stored at 4ºC.

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Sodium phosphate buffer - Cell Biology Protocols

11/20/2014
07:04 | Author: Devin Garcia

Oral sodium phosphate tablets
Sodium phosphate buffer - Cell Biology Protocols

0.1M Phosphate buffer chart. Stock solution A. 0.2 M monobasic sodium phosphate, monohydrate (27.6g/L) 0.2 M dibasic sodium phosphate (28.4 g/L).

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PHOSPHATE BUFFERS - PCC

9/19/2014
05:56 | Author: Evan Martin

Oral sodium phosphate tablets
PHOSPHATE BUFFERS - PCC

Phosphate buffer (sodium salt) is typically prepared by mixing solutions of sodium phosphate dibasic (Na2HPO4) and sodium phosphate monobasic.

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PH table for Potassium and Sodium Phosphate Buffers - UltraScan

7/18/2014
03:28 | Author: Caitlin White

Oral sodium phosphate tablets
PH table for Potassium and Sodium Phosphate Buffers - UltraScan

pH table for Potassium phosphate buffer at 25°C. pH, % K2HPO4 (dibasic), % KH2PO4 (monobasic). 5.8, 8.5, 91.5. 6.0, 13.2, 86.8. 6.2, 19.2.

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Sodium-phosphate-buffer - WikiFormulation

5/17/2014
01:20 | Author: Caitlin White

Dexamethasone sodium phosphate tablets
Sodium-phosphate-buffer - WikiFormulation

Sodium phosphate buffer is used routinely in the life sciences. Stock solution A: 1 M monobasic sodium phosphate, anhydrous, MW 119.98 (thoroughly Stock solution B: 1 M dibasic sodium phosphate, anhydrous, MW 141.96 (thoroughly.

Stock solution A: 1 M monobasic sodium phosphate, anhydrous, MW 119.98 (thoroughly mix 120.0 g/liter of water) Stock solution B: 1 M dibasic sodium phosphate, anhydrous, MW 141.96 (thoroughly mix 142 g/liter of water) note: I use 18 megaohm ddH20 water for critical applications. I use 18 megaohm distilled water for dilution. 1. Also, you may dilute this to a desired concentration with water and the pH should stay the same. Using the chart below, mix the appropriate volume of solutions A and B to achieve a 1M buffer solution at the desired pH: Volume of Solution A (ml), Volume of Solution B (ml), pH 92.0, 8.0, 5.8 90.0, 10.0, 5.9 87.7, 12.3, 6.0 85.5, 15.0, 6.1 81.5, 19.5, 6.2 77.5, 22.5, 6.3 73.5, 26.5, 6.4 68.5, 31.5, 6.5 62.5, 37.5, 6.6 56.5, 43.5, 6.7 51.0, 49.0, 6.8 45.0, 55.0, 6.9 39.0, 61.0, 7.0 33.0, 67.0, 7.1 28.0, 72.0, 7.2 23.0, 77.0, 7.3 19.0, 81.0, 7.4 16.0, 84.0, 7.5 13.0, 87.0, 7.6 10.5, 89.5, 7.7 8.5, 91.5, 7.8 For example, 45 mls of Sol A + 55 mls of Sol B = 1M sodium phosphate solution at pH 6.9. However, use Whatman or EMD pH indicator strips to double check the pH after dilution. Also note that the buffering capacity depends on the concentration of the sodium phosphate, so dilution will decrease buffering capacity. www.openonward.org.

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The protocol below is fairly straight forward and describes how to make buffered solutions at various pH's; I've used it successfully for PEGylation of proteins at pH 7.4 and a concentration of 50 mM. The relevant pKa for sodium phosphate is 6.82 (the other pKa's are 2.15 and 12.38; these are not significant in the pH range used here). Sodium phosphate buffer is used routinely in the life sciences. The recommended buffering range is pH 5.8 to 7.8.

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